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Ellie Rose Mattoon et al. have confirmed that twenty mushroom species (including Boletus separans and species from the genus Hortiboletus) maintain subambient temperatures in both their fruited bodies and their mycelium (the root-like hyphae from which the fruited bodies grow), though the benefits of this hypothermic behavior are unclear. Noting that relative coldness was more pronounced in fruiting than in nonfruiting sites of the mycelium—a difference that persisted even after fruited bodies were detached—the researchers speculate that thermoregulation in the mycelium may influence reproductive success.