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Some pharmaceuticals contain titanium dioxide nanoparticles (-NPs), which can leach into waterways and soils via wastewater. In a 2014 study, Cheng Tan and Wen-Xiong Wang found that -NPs can accumulate in the bodies of water fleas (Daphnia magna). While bioaccumulation of manufactured nanoparticles may be inherently worrisome, it has been hypothesized that -NP bioaccumulation in invertebrates like D. magna could serve a valuable proxy role, obviating the need for manufacturers to conduct costly and intrusive sampling of vertebrate species-such as Chinook salmon (Oncorhynchus tshawytscha), commonly used in regulatory compliance testing for nanoparticle bioaccumulation, as environmental protection laws currently require.