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Over time, marine mollusks called nautilids continually add segments to their shells, and the chemical composition of each segment is influenced by the available materials in the surrounding water when the segment forms. Paleontologist Amane Tajika and colleagues analyzed the septa (the walls between shell chambers) of one male (samples labeled "M") and one female (samples labeled "F") nautilid, finding that sample F07 had the same concentration of oxygen-18 as did water approximately 135 meters deep and that the oxygen-18 concentration in sample M28 matched that of water at approximately 365 meters deep. Tajika et al. concluded that sample F07 formed at a higher point in the water column than did M28.