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Firefly luciferase (Fluc) is a distinctly evolved enzyme that can oxidize a substrate called D-luciferin to induce bioluminescence and can act as a fatty acyl-CoA synthetase (ACS) enzyme, a class of enzyme present in all insects. _____ Fluc is a bifunctional enzyme whose presence indicates an insect is capable of emitting light; in contrast, the presence of ACS alone would be insufficient for determining bioluminescence.