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Some herbicides contain copper oxide nanoparticles (CuO-NPs), which can leach into waterways and soils via wastewater. In a 2012 study, Chengfang Pang and colleagues found that CuO-NPs can accumulate in the bodies of New Zealand mud snails (Potamopyrgus antipodarum). While bioaccumulation of manufactured nanoparticles may be inherently worrisome, it has been hypothesized that CuO-NP bioaccumulation in invertebrates like P. antipodarum could serve a valuable proxy role, obviating the need for manufacturers to conduct costly and intrusive sampling of vertebrate species—such as African clawed frogs (Xenopus laevis), commonly used in regulatory compliance testing for nanoparticle bioaccumulation, as environmental protection laws currently require.